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	Provedor de dados ArchiMer (2) Autor Gourmelon, Michele (2) Solecki, O. (2) Hervio-heath, Dominique (1) Jarde, E. (1) Jeanneau, L. (1) Marin, Charlotte (1) Marti, R. (1) Mieszkin, Sophie (1) Pourcher, A. -m. (1) Pourcher, A. M. (1) Mais... Palavra-chave Lactobacillus amylovorus (2) Bacteroidales marker (1) Biphasic kinetic (1) Decay rates (1) Faecal source tracking (1) Faecal stanols (1) Microbial source tracking (1) Oxygen (1) Persistence (1) Pig-associated Bacteroidales markers (1) Quantitative real-time PCR (1) River water (1) Temperature (1) Mais... Tipo do documento Text (2) Ano 2011 (2) País France (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms ArchiMer Marti, R.; Mieszkin, Sophie; Solecki, O.; Pourcher, A. -m.; Hervio-heath, Dominique; Gourmelon, Michele. Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log... Tipo: Text Palavras-chave: Lactobacillus amylovorus; Microbial source tracking; Oxygen; Persistence; Pig-associated Bacteroidales markers; Quantitative real-time PCR; River water; Temperature. Ano: 2011 URL: http://archimer.ifremer.fr/doc/00053/16470/14534.pdf Persistence of microbial and chemical pig manure markers as compared to faecal indicator bacteria survival in freshwater and seawater microcosms ArchiMer Solecki, O.; Jeanneau, L.; Jarde, E.; Gourmelon, Michele; Marin, Charlotte; Pourcher, A. M.. Natural seawater and freshwater microcosms inoculated with pig manure were set up to determine the persistence of pig faecal microbial and chemical markers in these two types of surface water. The concentrations of Lactobacillus amylovorus, the Bacteroidales Pig-2-Bac 165 rRNA genetic marker, five stanols and the evolution of two ratios of stanols, R(1) (coprostanol to the sum of coprostanol and 24-ethylcoprostanol) and R(2) (sitostanol to coprostanol) were analyzed during two months along with the concentration of Faecal Indicator Bacteria (FIB). Pig manure was inoculated to unfiltered water microcosms incubated aerobically at 18 degrees C in the dark. The faecal contamination load represented by the concentrations of culturable Escherichia coli and/or... Tipo: Text Palavras-chave: Faecal source tracking; Decay rates; Faecal stanols; Biphasic kinetic; Lactobacillus amylovorus; Bacteroidales marker. Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15417/12975.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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